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Effect of pH on the adherence, surface hydrophobicity and the biofilm formation of Gardnerella vaginalis

*, Udayalaxmi and Bhat, Gopalkrishna and Kotigadde, Subbannayya and Kotian, Shashidhar (2012) Effect of pH on the adherence, surface hydrophobicity and the biofilm formation of Gardnerella vaginalis. Journal of Clinical Diagnosis and Research, 6 (6). pp. 967-969.

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Abstract

Background: Bacterial vaginosis is a common cause of abnormal vaginal discharge in women of reproductive age. Although bacterial vaginosis is prevalent, not much progress has occurred in identifying the factors responsible and associated with bacterial vaginosis and its pathophysiology. Here we would like to evaluate the effect of the change in the pH of the vagina on the virulence factors of Gardnerella vaginalis the main organism indicated in this vaginal condition. Objective: The objective of the present study was to observe the effect of pH on the adherence, surface hydrophobicity and biofilm formation by 10 strains of Gardnerella vaginalis isolated from cases of bacterial vaginosis. Result: We found that adherence to vaginal epithelial cells and surface hydrophobicity was maximum at lower pH (pH 3,4,5) and minimum at higher pH (pH 6,7) but pH did not have significant effect on biofilm formation. Conclusion: Increase in pH of the vagina observed during bacterial vaginosis probably occurs much later during the disease. During the early part of the disease, the bacteria adhere to the vaginal epithelium, multiply in large numbers and form a thick biofilm which is not affected by the rise in pH which occurs probably due to the metabolic activities of this large bacterial population. This also explains the fact that all women with bacterial vaginosis do not have an elevated vaginal pH.

Item Type: Article
Uncontrolled Keywords: Adherence,Bacterial vaginosis, Biofilm, Gardnerella vaginalis, pH, Surface hydrophobicity
Subjects: Medicine > KMC Mangalore > Microbiology
Medicine > KMC Mangalore > Community Medicine
Depositing User: KMCMLR User
Date Deposited: 23 Aug 2012 05:01
Last Modified: 23 Aug 2012 05:01
URI: http://eprints.manipal.edu/id/eprint/77065

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