Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

Patil, Ajeetkumar and Choudhari, KS and Prabhu, Vijendra and Unnikrishnan, VK and Bhat, Sujatha and Pai, Keerthilatha M and Santhosh, C (2012) Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications. International Scholarly Research Network.

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Abstract

This paper describes the sensitivity study and performance evaluation of high-performance liquid chromatography-laser-induced fluorescence detection (HPLC-LIF) system assembled in our laboratory for proteomics applications. The limits of Detection (LOD) of several serum proteins have been estimated with this instrument and are found to be much lower compared to other commonly used proteomics techniques like SELDI, MALDI, 2-D-SDS-PAGE, and so forth. Techniques for improving the LOD still further with similar setup are briefly discussed. Using the system, protein profiles of serum in normal, malignant, and premalignant conditions were recorded for different malignancy situations.

Item Type: Article
Subjects: Dentistry > MCODS Manipal > Oral Medicine and Radiology
Departments at MU > Atomic Molecular Physics
Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Microbiology
Depositing User: KMC Manipal
Date Deposited: 26 Sep 2012 07:28
Last Modified: 26 Sep 2012 07:28
URI: http://eprints.manipal.edu/id/eprint/77272

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