Bacteriological Profile and Antibiogram of Blood Culture Isolates in a Pediatric Care Unit

Prabhu, Kavitha and Bhat, Sevitha and Rao, Sunil (2010) Bacteriological Profile and Antibiogram of Blood Culture Isolates in a Pediatric Care Unit. Journal of Laboratory Physicians, 2 (2). pp. 85-88.

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Abstract

Background & objectives:Septicemia is one of the important causes of mortality and morbidity in children. Blood culture is the gold standard for the diagnosis. Emergence of multidrug resistant bacterial strains is a major problem in the management of neonatal sepsis. Present study was undertaken to identify the common bacterial pathogens associated with neonatal sepsis and to determine their antibiotic susceptibility pattern. Materials and Methods: Blood cultures from185 suspected cases of neonatal sepsis were examined. The growth from the sub-cultures were identified by conventional biochemical tests.Antibiotic susceptibility testing was performed by Modified Kirby-Bauer disc diffusion method and drug resistant strains in primary screening were further processed for ESBL and MRSA status by combination disc method(ESBL) and Oxacillin disc diffusion method(MRSA). Results: Out of the 185 cultures obtained from suspected cases, 81(44%) were culture positive. Fifty two (35%) of the culture isolates were Gram negative bacilli.Twenty eight (64%) of the isolates were Gram positive cocci.One case was of mixed infection. The prevalence of MRSA in 41 strains of Staphylococcus aureus was found to be 29% (12 strains). The overall prevalence of ESBL producers among 28 Gram negative bacterial isolates was found to be 32%( 9 strains). Conclusion: This study stresses the need for the continuous screening and surveillance for antibiotic resistance in pediatric care unit.

Item Type: Article
Uncontrolled Keywords: Sepsis, methicillin resistant Staphylococcus aureus , extended spectrum beta lactamases, blood culture
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 03 Oct 2012 10:27
Last Modified: 03 Oct 2012 10:27
URI: http://eprints.manipal.edu/id/eprint/77330

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