Thakur, Goutam and Banerjee, Shubhadeep and Mitra, Analava and Basak, Amit (2012) Genipin Crosslinked Gelatin Hydrogel: Tolbutamide Release and Cytocompatibility. Journal of Tissue Engineering and Regenerative Medicine, 6 (1). p. 324. ISSN 1932-7005

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Abstract

Gelatin based drug carrier matrices have gained intense interest as a very promising class of delivery system. The aim of this study was to develop drug (tolbutamide) loaded gelatin based gels. Gelatin gel due to its reversible gel-sol transition at ~35°C and potentially low gel strength is often chemically crosslinked to improve its thermal and mechanical stability. Here, gelatin matrices were crosslinked with genipin, a naturally occurring crosslinker for the release of tolbutamide. Tolbutamide (anti-diabetic agent) was incorporated into the gelatin matrices to form drug loaded gel for the release study. Morphological analysis using confocal microscopy revealed network structure. The release of tolbutamide from the crosslinked gels indicated an initial increase upto 8 h. This was followed by a steady release state after 20 h (52%). Release kinetics was determined following Peppas model. The diffusional exponent (n) (0.84±0.01) (p<0.05) indicated anomalous release behaviour. Further, in vitro cellular compatibility and normal cell proliferation of AH-927 cell line was observed in live dead assay and fluorescence microscopy studies using propidium iodide staining. The studies supported that the matrices display excellent compatibility without compromising the cellular integrity and thus can be utilized as safe carrier matrices for drug transport.

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