Quantification of Sennosides By Reverse Phase High Performance Liquid Chromatography Coupled With Electro Spray Ionization Tandem Mass Spectrometry in Unani Formulations

Shreedhara, CS and Ghosh, A and Shenoy, G and Shetty, R and Kumar, VS and Sanghai, DB (2013) Quantification of Sennosides By Reverse Phase High Performance Liquid Chromatography Coupled With Electro Spray Ionization Tandem Mass Spectrometry in Unani Formulations. International Journal of Pharmacognosy and Phytochemical Research, 4 (4). pp. 224-226. ISSN 0975-4873

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Abstract

Unani system is a science which deals with the preventive and promotive aspects of health of human beings and health problems occurred by the Ecological and Environmental factors, which may vitiate humours i.e. Blood, Phlegm, Yellow bile and Black bile, the fluids circulating in the body vessels. There is considerable demand in drug testing for a specific and precise analytical method for the identification of sennosides in various Unani formulations. A combination of high performance liquid chromatography and mass spectrometry (LC-MS/MS) will provide unambiguous fingerprint information for chemical structural confirmation and estimation of sennosides. The objective of the present investigation is to develop a simple, economical and reliable high performance liquid chromatography method for the quantification of sennosides in Unani formulations. The validated method allows quantification of sennosides in 1 – 100.00 μg/mL. The correlation coefficient was ≥0.9990 for the sennosides. The simplicity of the assay and rapid liquid-liquid extraction make it an attractive procedure in estimation of sennosides in Unani formulations. The separation was achieved in C18 column and negative ion mode was used for detection in ESI-MS detection.

Item Type: Article
Uncontrolled Keywords: Sennosides; Hydrochlorothiazide and RPHPLC
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Depositing User: KMC Manipal
Date Deposited: 14 Jan 2013 05:11
Last Modified: 14 Jan 2013 05:11
URI: http://eprints.manipal.edu/id/eprint/77970

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