Ovarian development in Wistar rat treated prenatally with single dose diisobutyl phthalate

Ray, B and D’Souza, Antony Sylvan and Kumar, V and Pugazhandhi, B and D’Souza , MR and Nayak, D and Sushma, RK and Shetty, P and Singh, H and Krishna, L and Bhat, KMR and Rao, ACK and Kumar, N (2012) Ovarian development in Wistar rat treated prenatally with single dose diisobutyl phthalate. Bratisl Lek Listy, 113 (10). pp. 577-582.

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Phthalates are a class of industrial compounds with an array of toxicological properties used in day to day life. Diisobutyl phthalate on (DIBP) is used as an additive to keep the plastics soft or fl exible (plasticizer) in nitrocellulose plastic, nail polish, explosives, lacquer manufacturing etc. Although DIBP exposure in humans is generally low, people in adhesive industries and harmaceutical industries are exposed to higher levels. The aim of this study was to determine the effect of single dose of DIBP on developing ovary of Wistar rat. One hundred and eight adult pregnant Wistar rats were divided into control and experimental groups. Rats in experimental group were given DIBP on day 10, 12 and 14 of gestation at 0.375, 0.75 and 1.25 ml/kg body weight dose intraperitoneally in a single dose. Sections of ovaries collected on day 21 of gestation were stained with hematoxylin and eosin and examined and Masson’s trichrome histologically. Sections belonging to the control group showed the presence of oocytes in clusters separated by thin fi brous septa. Degeneration oocytes, empty follicles surrounded by follicular cells without gonocytes in the center were observed in ovarian stroma. Blood vessels in the ovarian stroma were prominent and congested. Around a bunch of follicles total architectural disarray was observed although on special staining fi brosis was not evident. As pregnant women are constantly exposed, effect of DIBP on ovary of a developing fetus would denote the long term consequence in future generations.

Item Type: Article
Uncontrolled Keywords: Ovary, rat, Diisobutyl phthalate, Ovarian follicle, Embryology, Gonocytes
Subjects: Medicine > KMC Manipal > Anatomy
Health Sciences > KMCIC
Medicine > MMMC Manipal > Anatomy
Depositing User: KMC Manipal
Date Deposited: 01 Feb 2013 06:46
Last Modified: 04 Dec 2013 06:53
URI: http://eprints.manipal.edu/id/eprint/78131

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