High Carbohydrate Diet with 30% Sucrose Solution: An Optimized Rodent Model for Simulating Human Insulin Resistance

Theerthahalli, Arun Sudheendra and Shenoy, Rekha R and Nayak, Pawan G and Mudgal, Jayesh and Kumar, Nitesh and Rao, Mallikarjuna C and Kutty, Gopalan N (2013) High Carbohydrate Diet with 30% Sucrose Solution: An Optimized Rodent Model for Simulating Human Insulin Resistance. Pharmacologia, 4 (3). pp. 157-163. ISSN 2044-4648

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Background: The development of an appropriate animal model that can mimic the actual human disease condition is an important consideration of a successful pharmacological experiment. Methods: To achieve type-2 diabetic conditions similar to that of human, we performed an optimization experiment, taking in to consideration the previously reported methods of inducing type-2 diabetes such as high fat diet, high sucrose diet and high calorie diet models with low dose Streptozotocin (STZ). We adopted High Carbohydrate Diet (HCD) model with some modifications. Post weaned male albino rats were chosen for the experiment and were fed HCD for six months prior to the experiment. The rats were later grouped, treated with sucrose and fructose solutions and STZ was administered at two different doses. The animals were subjected to OGTT. Plasma glucose, insulin, total cholesterol, triglycerides, AST, ALT levels were measured. The comparison was done with both normal pellet diet and HCD rats. Results: The findings demonstrated that HCD induced frank obesity with increased circulating TG levels. The low dose STZ treatment was not encouraging as significant mortality was observed with 35 mg kg-1 STZ and reversal to normoglycemia was observed with 20 mg kg-1 STZ. Conclusion: The group with sucrose supplementation simulated the conditions desirable to mimic human insulin resistance syndrome.

Item Type: Article
Uncontrolled Keywords: Glucose excursion, triglycerides, total cholesterol, insulin assay, albino rats
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 12 Feb 2013 07:26
Last Modified: 18 Oct 2013 09:32
URI: http://eprints.manipal.edu/id/eprint/78345

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