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Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity

Archana, PR and Rao, Nageshwar B and Ballal, Mamatha and Rao, Satish BS (2009) Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity. Mutation Research, 680. pp. 70-77. ISSN 1383-5718

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Abstract

The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25�g/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25�g/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25�g/ml TOH treatment before irradiation significantly decreased the percentage of radiation induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2•, ABTS•+ and OH• in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.

Item Type: Article
Uncontrolled Keywords: Antioxidants; Apoptosis; Clonogenicity; Radiation ;Thymol.
Subjects: Health Sciences > KMCIC
Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 25 Feb 2013 09:52
Last Modified: 25 Feb 2013 09:52
URI: http://eprints.manipal.edu/id/eprint/78708

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