Predictive and Prognostic Significance of Glutathione Levels and DNA Damage in Cervix Cancer Patients Undergoing Radiotherapy

Vidyasagar, MS and Kodali, Maheedhar and Saxena, Prakash PU and Upadhya, Dinesh and Bejadi, Manjunath Vadhiraja and Fernandes, Donald J and Rao, Satish BS (2010) Predictive and Prognostic Significance of Glutathione Levels and DNA Damage in Cervix Cancer Patients Undergoing Radiotherapy. International Journal of Radiation Oncology, Biology, Physics, 78 (2). pp. 343-349. ISSN 0360-3016

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Abstract

Purpose: To assess the predictive significance of serum glutathione (GSH) and tumor tissue DNA damage in the treatment of cervical cancer patients undergoing chemoradiotherapy. Methods and Materials: This study included subjects undergoing hysterectomy (for normal cervix tissue) and cervical cancer patients who underwent conventional concurrent chemoradiotherapy (cisplatin once per week for 5 weeks with concurrent external radiotherapy of 2 Gy per fraction for 5 weeks, followed by two applications of intracavitary brachytherapy once per week after 2 weeks’ rest). Blood was collected after two fractions, whereas both blood and tissues were collected after five fractions of radiotherapy in separate groups of subjects. Serum for total GSH content and tissues were processed for single-cell gel electrophoresis (SCGE) assay for DNA damage analysis. Clinical tumor radioresponse was assessed 2 months after the completion of treatment as complete responders (CR) (100% shrinkage), partial responders (PR) (>50%), and nonresponders (NR) (<50%). Results: Serum GSHcontent depleted significantly after a total dose of 4Gy and 10 Gyof radiotherapy with a single dose of cisplatin, which was significantly lesser in NR than of CR patients. Similarly, Olive Tail Moment, the index of DNA damage, indicated significantly higher values in the fifth fraction of radiotherapy (5-RT) than in pretreatment. The DNA damage after 5-RT in the NR subgroup was significantly lower than that of CR. Conclusions: Serum GSH analysis and tumor tissue SCGE assay found to be useful parameters for predicting chemoradioresponse prior to and also at an early stage of treatment of cervical cancers.

Item Type: Article
Uncontrolled Keywords: Cervical carcinoma; Glutathione; SCGE assay; Predictive assays.
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Radiotherapy and Oncology
Depositing User: KMC Manipal
Date Deposited: 25 Feb 2013 10:01
Last Modified: 11 Dec 2015 09:26
URI: http://eprints.manipal.edu/id/eprint/78710

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