Synthesis, characterization and in vitro cytotoxic properties of some new Schiff and Mannich bases in Hep G2 cells

Satyamoorthy, K (2011) Synthesis, characterization and in vitro cytotoxic properties of some new Schiff and Mannich bases in Hep G2 cells. Medicinal Chemistry Research, 20 (7). pp. 1024-1032.

[img] PDF
Already_Enterd_Shashi_C_Medicinal_Chemistry_Research-2011-20,_1024-1032.pdf - Published Version
Restricted to Registered users only

Download (273kB) | Request a copy

Abstract

A series of 5-substituted-4-amino-3-mercapto- 1,2,4-triazoles were synthesized and were treated with various 3-substituted pyrazole aldehydes to obtain a series of new Schiff bases (3a–l). Few of the selected Schiff bases were converted into Mannich bases by reaction with diphenylamine/morpholine in presence of formaldehyde in ethanol media (4a–e, 5a–e). These newly synthesized compounds were characterized by elemental analysis, IR, NMR and mass spectrometry studies. A comparative study on the cytotoxic activities of few selected Schiff and Mannich bases was done in HepG2 cells using MTT assay. Few of the screened Schiff bases, 3a, 3d, 3e, 3g and 3h showed dose dependent cytotoxic activity, 3a being the most potent with an IC50 value of 0.018 g/l comparable to the standard drug doxorubicin. Among the Mannich bases, 5b was the most active with an IC50 value of 0.034 g/l. The Schiff bases were found to be more active, when compared to Mannich bases derived from them. The morpholinederived Mannich bases were more potent than those obtained from diphenyl amine.

Item Type: Article
Uncontrolled Keywords: 1,2,4-Triazoles ; Schiff bases ; Mannich bases ; HepG2 cell line ; MTT assay ; Cytotoxic activity.
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 27 Feb 2013 06:24
Last Modified: 24 Dec 2013 09:25
URI: http://eprints.manipal.edu/id/eprint/78754

Actions (login required)

View Item View Item