Antagonistic effects of Zingerone, a phenolic alkanone against radiation-induced cytotoxicity, genotoxicity, apoptosis and oxidative stress in Chinese hamster lung fibroblast cells growing in vitro

Rao, Satish BS and Rao, Nageshwar B (2010) Antagonistic effects of Zingerone, a phenolic alkanone against radiation-induced cytotoxicity, genotoxicity, apoptosis and oxidative stress in Chinese hamster lung fibroblast cells growing in vitro. Mutagenesis, 25 (6). pp. 577-587.

[img] PDF
B.S._Satish_Rao_-_Mutagenesis-2010_(63).pdf - Published Version
Restricted to Registered users only

Download (656kB) | Request a copy

Abstract

Zingerone (ZO), a dietary phenolic compound was investigated for its ability to protect against radiationinduced oxidative stress and DNA damage in Chinese hamster fibroblast cells (V79). Cells treated with optimal dose of ZO (25 mg/ml), 1 h prior radiation exposure resulted in a significant (P < 0.01) elevation of cell survival and decreased the genotoxicity (micronuclei and comet assays). Further, pretreatment with ZO significantly reduced radiation-induced oxidative stress as indicated by decreased reactive oxygen species levels and inhibition of mitochondrial depolarisation. The experiments conducted to evaluate the intracellular antioxidant activity in ZO-pretreated cells demonstrated a significant (P < 0.01) increase in the various antioxidants like glutathione, gluthione-S-transferase, superoxide dismutase, catalase and a significant (P < 0.01) decrease in malondialdehyde levels versus irradiation alone. Further, ZO scavenged various free radicals generated in vitro (OH�, O2�, DPPH�, ABTS�1 and NO�) in a dose-dependent manner. The antiapoptotic effect of ZO pretreatment was by the inhibition of the activation of capase-3, by upregulating Bcl-2 and down-regulating Bax proteins. Our study demonstrates the antagonistic effect of ZO against radiationinduced cytotoxicity. Further, ZO rendered anti-genotoxic, anti-apoptotic and anti-lipid peroxidative potency, plausibly ascribable to its antioxidant/free radical scavenging ability and also by the suppression of radiation-induced oxidative stress.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 27 Feb 2013 10:57
Last Modified: 27 Feb 2013 10:57
URI: http://eprints.manipal.edu/id/eprint/78775

Actions (login required)

View Item View Item