Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from TanneryWasteWater

Raja, Selvaraj and Ramachandra Murty, V (2012) Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from TanneryWasteWater. International Scholarly Research Network. pp. 1-9.

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Abstract

Aqueous two phase systems (ATPSs) containing high molecular weight polyethylene glycol, PEG (10000), and completely biodegradable citrate salts (sodium citrate, potassium citrate, and ammonium citrate) with water were developed to recover the waste water proteins from a model tannery waste water system. The variations in the phase diagram were explained on the basis of ionic radius of cations, Gibbs free energy of hydration (ΔGhydation) of cations, and effective excluded volume (EEV) of salts. The salting-out capability of the cations followed the sequence: Sodium citrate > Potassium citrate > Ammonium citrate. Setschenow-type equations were used to correlate tie-line compositions. During partitioning studies, recovery of tannery waste water proteins in PEG 10000 + Sodium citrate ATPS was found superior to other systems. It was possible to recover 95.86% of proteins from tannery waste water in the bottom phase with PEG 10000 30% (w/w) + Sodium citrate 13% (w/w) at 30◦C. The partition coefficients were correlated with the salt compositions by a quadratic equation and the coefficients were calculated.

Item Type: Article
Subjects: Engineering > MIT Manipal > Biotechnology
Engineering > MIT Manipal > Chemical
Depositing User: MIT Library
Date Deposited: 18 Apr 2013 07:16
Last Modified: 23 Sep 2015 15:12
URI: http://eprints.manipal.edu/id/eprint/79743

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