Verma, Pankaj and Seong, Chi Nam and Pandey, Prashant Kumar and Bhonde, Ramesh Ramchandra and Spröer, Cathrin and Rohde, Manfred and Shouche, Yogesh Shreepad (2013) Bhargavaea indica sp.nov., a Member of the Phylum firmicutes,Isolated from Arabian Sea Sediment. Journal of Microbiology., 51 (1). pp. 36-42.

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Abstract

A Gram-positive, aerobic, coccoid-rod shaped, non-motile, catalase- and oxidase-positive bacterium, designated train KJW98T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β- aemolytic, non-endospore-forming and grew with 0–8.5% (w/v) NaCl, at 15–48°C and at pH 6.5–9.0, with optimum growth with 0.5% (w/v) NaCl, at 42°C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and gyrB gene equences showed that strain KJW98T forms a lineage within the genus Bhargavaea. The G+C content of the genomic DNA as 55 mol%. The DNA-DNA relatedness values of strain JW98T with B. beijingensis DSM 19037T, B. cecembensis LMG 24411T and B. ginsengi DSM 19038T were 43.2, 39 and 26.5%, respectively. The major fatty acids were anteiso-C15:0 37.7%), iso-C15:0 (19.7%), anteiso-C17:0 (17.0%) and iso-C16:0 (11.1%). The predominant menaquinone was MK-8 and the cell-wall peptidoglycan was of A4α type with L-lysine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The phenotypic, genotypic and DNA-DNA relatedness data ndicate that strain KJW98T should be distinguished from the members of the genus Bhargavaea, for which the name Bhargavaea indica sp. nov. is proposed with the type strain KJW98T (=KCTC 13583T =LMG 25219T).

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