Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications.

Mamidi, Murali Krishna and Singh, Gurbind and Husin, Juani Mazmin and Nathan, Kavitha Ganesan and Sasidharan, Gopinath and Zakaria, Zubaidah and Bhonde, Ramesh R and Majumdar, Anish Sen and Das, Anjan Kumar (2012) Impact of passing mesenchymal stem cells through smaller bore size needles for subsequent use in patients for clinical or cosmetic indications. Journal of Translational Medicine., 10 (229).

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Abstract

Background:Numerous preclinical and clinical studies have investigated the regenerative potential and the trophic support of mesenchymal stem cells (MSCs) following their injection into a target organ. Clinicians favor the use of smallest bore needles possible for delivering MSCs into vascular organs like heart, liver and spleen. There has been a concern that small needle bore sizes may be detrimental to the health of these cells and reduce the survival and plasticity of MSCs. Methods:In this report, we aimed to investigate the smallest possible bore size needle which would support the safe delivery of MSCs into various tissues for different clinical or cosmetic applications. To accomplish this we injected cells via needle sizes 24, 25 and 26 G attached to 1 ml syringe in the laboratory and collected the cells aseptically. Control cells were ejected via 1 ml syringe without any needle. Thereafter, the needle ejected cells were cultured and characterized for their morphology, attachment, viability, phenotypic expression, differentiation potential, cryopreservation andin vivo migration abilities. In the second phase of the study, cells were injected via 26 G needle attached to 1 ml syringe for 10 times. Results:Similar phenotypic and functional characteristics were observed between ejected and control group of cells. MSCs maintained their cellular and functional properties after single and multiple injections. Conclusions:This study proves that 26 G bore size needles can be safely used to inject MSCs for clinical/ therapeutics purposes.

Item Type: Article
Additional Information: Copy right of this article is belongs to BioMed Central.
Uncontrolled Keywords: Bone Marrow Mesenchymal stem cells (BM-MSC),Differentiation,Cell migration needle bore size,MSC transplantation/infusion.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 30 Apr 2013 10:33
Last Modified: 30 Apr 2013 10:33
URI: http://eprints.manipal.edu/id/eprint/79885

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