An in vitro evaluation of the antibacterial effect of calcium hydroxide and chlorhexidine on enterococcus faecalis

Tina, PP (2011) An in vitro evaluation of the antibacterial effect of calcium hydroxide and chlorhexidine on enterococcus faecalis. Guident, 5. pp. 55-59.

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Abstract

Aims & Objectives : To evaluate the antibacterial activity of Calcium hydroxide and Chlorhexidine on Enterococcus faecalis. Materials & Methods: Mueller- Hinton agar plates were swabbed with Enterococcus faecalis and sterile Whatmann No. 1 filter paper disks which were saturated with the test agents were placed on the plates paper disks were impregnated with: (1) Calcium hydroxide powder with Saline (2) Calcium hydroxide powder with Propylene glycol (3) Calcium hydroxide paste(pulpdent) (4) 0.12% Chlorhexidine solution (5) 0.12% Chlorhexidine solution with Calcium hydroxide powder (6) Ampicillin (positive control).. The plates were incubated at 370 C for 48 hours. Antibacterial actions of these groups were tested by Kirby Bauers disks diffusion technique. Kruskal Wallis and Mann Whitney ‘U’ test was performed to analyze the differences between the groups. Results: Ampicillin showed maximum zone of inhibition followed by 0.12% Chlorhexidine solution and combination of Calcium hydroxide and 0.12% Chlorhexidine respectively.No statistically significant difference was found between 0.12% Chlorhexidine solution and combination of 0.12% Chlorhexidine and Calcium hydroxide Conclusion: Present study shows that 0.12% Chlorhexidine solution has the maximum antibacterial effect against Enterococcus faecalis.

Item Type: Article
Uncontrolled Keywords: Calcium hydroxide; Chlorhexidine ;enterococcs faecalis
Subjects: Dentistry > MCODS Manipal > Conservative Dentistry and Endontics
Depositing User: KMC Manipal
Date Deposited: 02 May 2013 04:38
Last Modified: 02 May 2013 04:38
URI: http://eprints.manipal.edu/id/eprint/79927

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