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Evaluation of semiquantitative culture method in the diagnosis of central venous intravascular catheter related infections

Amin, Nirma.S. and *, Nilica Devi Sh and Bhat, Sevitha (2013) Evaluation of semiquantitative culture method in the diagnosis of central venous intravascular catheter related infections. Journal of Pharmaceutical and Biomedical Sciences, 29 (29). pp. 724-728. ISSN 2230 – 7885

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Abstract

Background & objectives: The use of intravascular devices frequently is complicated by a variety of local or systemic infectious complications, including septic thrombophlebitis, endocarditis, bloodstream infection (BSI). Catheter- related infection is defined according to catheter-tip colonization, catheter related local infection (CRLI) and catheter related blood stream infection (CRBSI). The semiquantitative culture technique is useful in the diagnosis of bacteremia associated with central venous catheters. The present study was undertaken to evaluate the usefulness of semiquantitative culture of catheter tips of Central venous catheters. Materials and Methods: The cross sectional study was conducted in the Department of Microbiology, KMC, Mangalore. This study included 63 catheter tips and blood culture samples from patients with suspected intravascular catheter related infection. Antibiotic susceptibility testing was performed by Modified Kirby-Bauer disc diffusion method. Results: The overall tip colonization was 32 (50.79%) had growth >15 CFU and 31 (49.2%) had no growth. Out of the 32, definite catheter associated bacteremia was 5(7.93%), catheter associated infection was 18(28.57%), probable catheter associated bacteremia was 9(14.28%). The organisms isolated were Staphylococcus aureus, Enterococcus spp, Streptococcus pneumoniae, E.coli, Klebsiella spp, Citrobacter spp, Acinetobacter spp, Candida albicans& C. tropicalis. Conclusion: There is significant lack of specificity (a high number of false-positive results) of the Semi quantitative technique for the diagnosis of CRBSI , while its sensitivity is probably satisfactory.

Item Type: Article
Uncontrolled Keywords: Catheter related sepsis , HAI , Device associated infection
Subjects: Medicine > KMC Mangalore > Microbiology
Depositing User: KMCMLR User
Date Deposited: 10 May 2013 06:43
Last Modified: 10 May 2013 06:43
URI: http://eprints.manipal.edu/id/eprint/79949

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