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Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

Ramesh, Vinayagam and Ramachandra Murty, V (2014) Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352. Enzyme Research.

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Abstract

Glucoamylase is an industrially important enzyme which converts soluble starch into glucose. The media components for the production of glucoamylase from thermophilic fungus Humicola grisea MTCC 352 have been optimized. Eight media components, namely, soluble starch, yeast extract, KH2PO4, K2HPO4, NaCl, CaCl2, MgSO4 ⋅7H2O, and Vogel’s trace elements solution, were first screened for their effect on the production of glucoamylase and only four components (soluble starch, yeast extract, K2HPO4, and MgSO4 ⋅7H2O) were identified as statistically significant using Plackett-Burman design. It was fitted into a first-order model (

Item Type: Article
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 20 May 2015 07:25
Last Modified: 20 May 2015 07:25
URI: http://eprints.manipal.edu/id/eprint/142698

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