In Vitro Anticancer Activity Of Quercetin Isolated From Carmona retusa (Vahl.) Masam on Human Hepatoma Cell Line (Hepg2)

Chandrappa, C P and Govindappa, M and Kumar, Anil N V and Channabasava, R and Sadananda, T S and Sharanappa, P (2014) In Vitro Anticancer Activity Of Quercetin Isolated From Carmona retusa (Vahl.) Masam on Human Hepatoma Cell Line (Hepg2). IOSR Journal of Pharmacy and Biological Sciences, 9 (6). pp. 85-91. ISSN 2319-7676

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Abstract

Quercetin was isolated from an ethanol extract of Carmona retusa (Vahl.) Masam and was analyzed for its anticancer activity on HepG2 cell lines by MTT assay, Hoechst 33342 staining and Caspase-3 colorimetric assay. Plant extract yield of flavonoid quercetin from Carmona retusa (Vahl.) Masam showed significant and concentration-dependent anticancer activity at 100 μg/ml and 80 μg/ml doses after 24 and 48 h of treatment on HepG2 cell line in MTT assay, significant cell apoptosis have shown at 53μg/ml concentration of extract yield of flavonoid quercetin from Carmona retusa (Vahl.) Masam in Hoechst 33342 staining and a significant activation of caspase-3 observed at 100 μg/ml of extract yield of flavonoid quercetin from Carmona retusa (Vahl.) Masam after 24 h and even 48 h of incubation. Our findings suggesting that the plant extract yield of flavonoid quercetin from Carmona retusa (Vahl.) Masam could be used as a promising anticancer agent.

Item Type: Article
Uncontrolled Keywords: Carmona retusa, anticancer, HepG2 cell lines, MTT assay, Hoechst 33342, Caspase-3 colorimetric assay
Subjects: Engineering > MIT Manipal > Chemistry
Depositing User: MIT Library
Date Deposited: 21 Dec 2015 11:14
Last Modified: 21 Dec 2015 11:14
URI: http://eprints.manipal.edu/id/eprint/144895

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