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Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus

Chatterjee, Poonam Mishra and Silpi, Datta and Tiwari, Deepika Pandey and Ritu, Ravala and Ashok Kumar, Dubey (2018) Selection of an Effective Indicator for Rapid Detection of Microorganisms Producing γ-Polyglutamic Acid and Its Biosynthesis Under Submerged Fermentation Conditions Using Bacillus methylotrophicus. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 185 (01). pp. 270-288. ISSN 0273-2289

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Abstract

γ-Polyglutamic acid (γ-PGA) is a biosynthetic outcome of glutamic acid polymerization by microbes. In the current study, we have isolated Bacillus methylotrophicus on solid differential media containing methylene blue. This is the first report mentioning the use of methylene blue to distinguish the monomeric and polymeric form of glutamic acid in the liquid medium using UV-Vis spectrophotometer. Our method can simplify the analytical process of γ-PGA confirmation using the aforementioned studies. This screening protocol is sensitive to the detection of γ-PGA quantities as low as 3 μg/mL; thus, the potent producers can be effectively screened. Furthermore, we have carried out process optimization of the present strain for γ-PGA production wherein we could obtain 1.4-fold improvement in the yield with respect to utilization of carbon source and 2.6-fold increase with respect to nitrogen source under submerged fermentation at a shake flask level. We have shown an increase in γ-PGA titer from 1.5 to 36 g/L using mannitol, monosodium glutamate, peptone, and tween 20.

Item Type: Article
Uncontrolled Keywords: ?-Polyglutamic acid (?-PGA) . Bacillus methylotrophicus . Rapid screening method . Methylene blue .
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 03 Jul 2018 04:32
Last Modified: 03 Jul 2018 04:32
URI: http://eprints.manipal.edu/id/eprint/151452

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