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Screening of chitin deacetylase producing microbes from marine source using a novel receptor on agar plate

Pawaskar, Goutam Mohan and Pangannaya, Srikala and Raval, Keyur and Trivedi, Darshak R and Raval, Ritu (2019) Screening of chitin deacetylase producing microbes from marine source using a novel receptor on agar plate. International Journal of Biological Macromolecules, 131 (1). pp. 716-720. ISSN 0141-8130

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Abstract

Chitosan is a deacetylated formof naturally occurring polymer; chitin. On an industrial scale, the deacetylation of chitin to chitosan is performed using harsh chemicals like sodium hydroxide. This not only adds to the environmental pollution but the product is also randomin terms of its deacetylation. This shortcoming can be addressed by using enzymes like chitin deacetylase (CDA). The screening of these organisms would require a reliable, fast and sensitive screening method. The deacetylation of chitin into chitosan, releases acetate as the byproduct of the reaction. A receptor which specifically binds to the acetate ion was synthesized chemically. The receptor upon binding with the acetate ion emitted a fluorescence which could be viewed using the gel documentation unit. The receptorwas optimized for the screening of CDA producing microbes with the positive fungal control as Penicillium sp. and bacterial control as Bacillus megaterium. A parallel study with the 4-Nitroacetanilide, the reported screening indicator for CDA was performed. The results obtained with the receptor in the present study were concordant with the 4-Nitroacetanilide. Upon standardization, the protocol was extended for the screening of CDA producing microbes from the marine crustacean dumped soil and water samples. The CDA activity of these microbes was further confirmed using spectrophotometric MBTH assay. This is the first report using this receptor for the screening of CDA producers. Themethod is not only sensitive but also reproducible and can be extended for a high throughput screening of CDA producers.

Item Type: Article
Uncontrolled Keywords: chitin deacetylase, receptor, agar plate
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 15 Apr 2019 05:14
Last Modified: 15 Apr 2019 05:14
URI: http://eprints.manipal.edu/id/eprint/153645

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