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Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats

Nagamma, Takkella and Konuri, Anjaneyulu and Nayak, Chandrika D and Kamath, Shobha U and Udupa, Padmanabha EG and Nayak, Yogendra (2019) Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats. Journal of Taibah University Medical Sciences, 14 (4). pp. 383-389. ISSN 1658-3612

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Abstract

Objective: The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods: Female Wistar rats were allocated into five groups (n ¼ 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/ day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results: Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count,Hb levels, haematocrit, andMCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly (p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion: The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFDinduced obesity.

Item Type: Article
Uncontrolled Keywords: BMI; Fenugreek seed extract; Haematological parameters; High-fat diet; Lipid profile.
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Biochemistry
Medicine > MMMC Manipal > Biochemistry
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Library
Date Deposited: 23 Aug 2019 09:07
Last Modified: 23 Aug 2019 09:07
URI: http://eprints.manipal.edu/id/eprint/154437

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