4-({(1E)-[3-(4-fluorophenyl)-1H-pyrazol-4-yl]methylene}amino)-5-[(2- methylphenoxy)methyl]-4H-1,2,4-triazole-3-thiol as a Potent Anticancer Agent Against Hepatocellular Carcinoma

Sunil, Dhanya and Isloor, Arun M and Shetty, Prakash and Satyamoorthy, K (2011) 4-({(1E)-[3-(4-fluorophenyl)-1H-pyrazol-4-yl]methylene}amino)-5-[(2- methylphenoxy)methyl]-4H-1,2,4-triazole-3-thiol as a Potent Anticancer Agent Against Hepatocellular Carcinoma. International Conference on Biomedical Engineering and Technology, 11. pp. 183-186.

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Abstract

In the present study, MTT assay was conducted to measure the cytotoxicity of three newly synthesized Schiff bases, 4-({(1E)-[3-(4-chlorophenyl)-1H-pyrazol-4-yl]methylene}amino)-5-[(4-methylphenoxy)methyl]-4H-1,2,4-triazole-3-thiol (SB-1), 4-({(1E)-[3-(4-fluorophenyl)-1H-pyrazol-4- yl]methylene}amino)-5-[(4-methylphenoxy)methyl]-4H-1,2,4-triazole-3-thiol (SB-2) and 4-({(1E)-[3-(4- fluorophenyl)-1H-pyrazol-4-yl]methylene}amino)-5-[(2-methylphenoxy)methyl]-4H-1,2,4-triazole-3-thiol (SB-3). All three compounds exhibited a dose dependent cytotoxic effect with low IC50 values on hepatocellular carcinoma cell line, HepG2. The effect of the three Schiff bases on DNA synthesis was monitored by labeling cells using [3H] thymidine. Incorporation of [3H] thymidine in conjunction with cell cycle analysis suggested that SB-3 inhibited the growth of HepG2 cells. The appearance of apoptotic sub-G1 cells was detected using flow cytometry studies and the apoptotic action of SB-3 was confirmed through chromatin condensation studies by Hoechst staining.

Item Type: Article
Uncontrolled Keywords: Anticancer, Apoptosis
Subjects: Engineering > MIT Manipal > Chemistry
Life Sciences > MLSC Manipal
Engineering > MIT Manipal > Printing and Media
Depositing User: MIT Library
Date Deposited: 20 Feb 2013 05:42
Last Modified: 25 Oct 2013 06:56
URI: http://eprints.manipal.edu/id/eprint/78617

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