Cytotoxicity studies of microwave assisted natural products extracts in HeLa and MCF-7 cell lines

Sunil, Dhanya and Kumar, Anil N V and Nayak, Anand Shivaram and Raj, Sachin and Prabhu, Deepa and Ravikiran, . (2013) Cytotoxicity studies of microwave assisted natural products extracts in HeLa and MCF-7 cell lines. International Journal of Medicinal and Aromatic Plants, 3 (1). pp. 27-31. ISSN 2249 – 4340

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Abstract

Microwave assisted extraction of leaves and stems of seven tropical plants viz. Ficus hispida, Andrographis paniculata, Alstonia scholaris, Aporusa lindlyeyana, Melastoma malabathicum, Adenon indicum and Alternanthera dentate was carried out. Optimum extraction conditions were 600 W microwave power, 6 min extraction time (spread over two extraction cycles of 3 min each) and 10 min pre-leaching time using powdered stem and leaves samples. The two solvents employed were 90% ethanol and hexane with 10:1 (mL/g) as the solvent to material loading ratio. The residues obtained were screened for their cytotoxic activity by MTT assay in HeLa and MCF-7 cell lines. The ethanol extract of F. hispida (Moraceae) and A. scholaris (Apocyanaceae) leaves exhibited excellent cytotoxic activity with an IC50 value of 8.47µg/mL in HeLa and 10.23 µg/mL in MCF-7 cell lines, respectively. The alcoholic leaf extract of A. sholaris showed IC50 values of 20.62 µg/mL and 18.50 µg/mL in HeLa and MCF-7 cells respectively.

Item Type: Article
Additional Information: Copyright © Open Access Science Research Publisher
Uncontrolled Keywords: Microwave assisted extraction, cancer, cytotoxicity, MTT assay
Subjects: Engineering > MIT Manipal > Chemistry
Depositing User: MIT Library
Date Deposited: 18 Apr 2013 07:12
Last Modified: 18 Apr 2013 07:12
URI: http://eprints.manipal.edu/id/eprint/79736

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