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Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology

Raja, Selvaraj and Ramachandra Murty, V (2013) Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology. Journal of Engineering.

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Abstract

Aqueous two-phase system(ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodiumcitrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater. A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery. From this strategy, a secondorder polynomial model was obtained for the protein recovery and it was validated.The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33∘C, respectively, for a phase system composed of 20% (w/w) PEG 6000-15% (w/w) SC.Thus the proposed ATPS can serve as an alternative to the conventional precipitation method to recover the soluble proteins from tannery wastewater.

Item Type: Article
Subjects: Engineering > MIT Manipal > Biotechnology
Depositing User: MIT Library
Date Deposited: 25 Apr 2013 11:24
Last Modified: 16 Nov 2013 05:45
URI: http://eprints.manipal.edu/id/eprint/79876

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